{"product_id":"omsk-hemorrhagic-fever-virus-e-protein-ectodomain-polyclonal-antibody-ncp0151p","title":"Omsk Hemorrhagic Fever Virus E Protein Ectodomain Polyclonal Antibody - NCP0151P","description":"\u003cp\u003eOmsk Hemorrhagic Fever Virus E Protein Ectodomain Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: NCP0151P-50, NCP0151P-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: NC_005062.1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: OHFV-E\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:2000\u003cbr\u003e\nELISA: 1:5000~10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21NS1) but not in naïve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z' value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Omsk hemorrhagic fever virus E protein ectodomain polyclonal antibody detects endogenous levels of OHFV-E protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of OHFV-E.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892009636019,"sku":"NCP0151P-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892009668787,"sku":"NCP0151P-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_886946bf-044a-494c-876c-2f85ed0abd40.jpg?v=1702469146","url":"https:\/\/afsbio.com\/products\/omsk-hemorrhagic-fever-virus-e-protein-ectodomain-polyclonal-antibody-ncp0151p","provider":"AFSBio Inc.","version":"1.0","type":"link"}