{"product_id":"p116-rip-polyclonal-antibody-bs62404","title":"p116 Rip Polyclonal Antibody-BS62404","description":"\u003cp\u003ep116 Rip Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62404-50, BS62404-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q6WCQ1\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB  IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000  IHC:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS\/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho\/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The protein was purified from E.coli and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p116 Rip polyclonal antibody detects endogenous levels of p116 Rip protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p116 Rip polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:PC12 whole cell lysate\u003cbr\u003e\nLaneB:MEF whole cell lysate\u003cbr\u003e\nLaneC:A549 whole cell lysate\u003cbr\u003e\nLaneD:A2780 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 116 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human p116 Rip.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910456578227,"sku":"BS62404-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910456610995,"sku":"BS62404-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_83031e6c-651b-4b90-8dea-6a860b0c168b.jpg?v=1703092765","url":"https:\/\/afsbio.com\/products\/p116-rip-polyclonal-antibody-bs62404","provider":"AFSBio Inc.","version":"1.0","type":"link"}