{"product_id":"p47-phox-phospho-s370-polyclonal-antibody-bs4852","title":"p47-phox (phospho-S370) Polyclonal Antibody-BS4852","description":"\u003cp\u003ep47-phox (phospho-S370) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4852-50, BS4852-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P14598\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003ep47-phox (phospho-S370) Polyclonal Antibody-BS4852\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC ICC IP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nICC: 1:50~1:200\u003cbr\u003e\nIP: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-p47-phox (S370) polyclonal antibody detects endogenous levels of p47-phox protein only when phosphorylated at Ser370.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-p47-phox (S370) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: Hela cell treated with serum starvation (24h) \u003cbr\u003e\n Lane 2: Raw264.7 cell treated with serum starvation (24h) \u003cbr\u003e\n Lane 3: H9C2 cell treated with serum starvation (24h), Immunohistochemistry (IHC) analyzes of p-p47-phox (S370) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 370.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957294239923,"sku":"BS4852-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957294272691,"sku":"BS4852-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8e398519-325f-49f0-ab12-1c88ec8bf8c5.jpg?v=1705144481","url":"https:\/\/afsbio.com\/products\/p47-phox-phospho-s370-polyclonal-antibody-bs4852","provider":"AFSBio Inc.","version":"1.0","type":"link"}