{"product_id":"p53-acetyl-k386-polyclonal-antibody-bs64033","title":"p53 (Acetyl-K386) Polyclonal Antibody - BS64033","description":"\u003cp\u003ep53 (Acetyl-K386) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 100ug, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS64033-100ug, BS64033-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregμlates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regμlator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregμlated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Cellμlar tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Acetylated\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to residues in Human TP53 around the acetylated site of Lys386.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Ac-p53 (K386) polyclonal antibody detects endogenous levels of human p53 protein only when acetylated at Lys386. This antibody also recognizes mouse and rat p53 protein when acetylated at the corresponding residues.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 53 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Regμlation of Apoptosis, Hypoxia Signaling, Warburg Effect, Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint, Examples of Crosstalk Between Post-translational Modifications, Cell Cycle G2 M DNA Damage Signaling Pathway,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P04637\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"100ug","offer_id":45946342736051,"sku":"BS64033-100ug","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946342768819,"sku":"BS64033-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/p53-acetyl-k386-polyclonal-antibody-bs64033","provider":"AFSBio Inc.","version":"1.0","type":"link"}