{"product_id":"p53-mouse-monoclonal-antibody-mb9015","title":"p53 Mouse Monoclonal Antibody-MB9015","description":"\u003cp\u003ep53 Mouse Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9015-50, MB9015-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse Ig2b, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04637\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000 \u003cbr\u003e\nIHC\/ICC: 1:100-1:200 \u003cbr\u003e\nFC: 1:100-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of p53 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 53,43 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p53 Mouse mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: 293T whole cell lysate\u003cbr\u003e\n Lane 2: A431 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin., ICC staining p53 inA431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of p53.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044036542643,"sku":"MB9015-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044036575411,"sku":"MB9015-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_147fdd4a-7f5c-42e9-beb1-f6b1ed56ccf9.jpg?v=1707738844","url":"https:\/\/afsbio.com\/products\/p53-mouse-monoclonal-antibody-mb9015","provider":"AFSBio Inc.","version":"1.0","type":"link"}