{"product_id":"p53rfp-polyclonal-antibody-e-ab-15118","title":"p53RFP Polyclonal Antibody - E-AB-15118","description":"\u003cp\u003ep53RFP Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-15118-60, E-AB-15118-120, E-AB-15118-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: RNF144B\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: bA528A10.3; E3 ubiquitin protein ligase RNF144B; E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain containing protein 2; IBR domain-containing protein 2; IBRDC 2; IBRDC2; KIAA0161; MGC71786; OTTHUMP00000016079; OTTHUMP00000016080; OTTHUMP00000039360; p53 inducible RING finger protein; p53-inducible RING finger protein; p53RFP; PIR2; R144B; Ring finger 144B; RING finger protein 144B; RNF 144B; RNF144B\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell Biology\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n \n\n\u003cp\u003eUNIProt ID: Q7Z419\u003c\/p\u003e\n\n\u003cp\u003eBackground: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 0.4 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein of human RNF144B\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:200-1:1000, IHC 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 34kDa\u003c\/p\u003e\n\n \n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43141468324019,"sku":"E-AB-15118-60","price":200.2,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43141468356787,"sku":"E-AB-15118-120","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43141468389555,"sku":"E-AB-15118-200","price":558.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_3a6e768f-5b5c-4f68-95eb-7e72f1aadda6.png?v=1710156214","url":"https:\/\/afsbio.com\/products\/p53rfp-polyclonal-antibody-e-ab-15118","provider":"AFSBio Inc.","version":"1.0","type":"link"}