{"product_id":"pak4-5-6-v469-polyclonal-antibody-bs3247","title":"PAK4\/5\/6 (V469) Polyclonal Antibody-BS3247","description":"\u003cp\u003ePAK4\/5\/6 (V469) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3247-50, BS3247-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O96013\/Q9NQU5\/Q9P286\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIP: 1:10~1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42\/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PAK4\/5\/6 (V469) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 64 kDa (PAK4), 80 kDa (PAK5), 75 kDa (PAK6)\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PAK4\/5\/6 (V469) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: H1792 whole cell lysate (20ug)\u003cbr\u003e\n Lane 2: MCF-7 whole cell lysate (20ug)\u003cbr\u003e\n Lane 3: PC3 whole cell lysate (20ug)\u003cbr\u003e\n Lane 4: The Testis tissue lysate of Mouse (40ug)\u003cbr\u003e\n Lane 5: The Testis tissue lysate of Rat (40ug), Immunoprecipitation of HEK293T cell lysate using PAK4\/5\/6 (V469) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using PAK4\/5\/6 (V469) #BS3247. “↑”（supernatant）;“↓(deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine\/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine\/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989553877171,"sku":"BS3247-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989553909939,"sku":"BS3247-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_20c3ae2a-7331-49a9-8516-a1d8ae1a2ffd.jpg?v=1706095255","url":"https:\/\/afsbio.com\/products\/pak4-5-6-v469-polyclonal-antibody-bs3247","provider":"AFSBio Inc.","version":"1.0","type":"link"}