{"product_id":"pan-mono-methyl-lysine-polyclonal-antibody-bs74114","title":"Pan Mono - Methyl Lysine Polyclonal Antibody - BS74114","description":"\u003cp\u003ePan Mono-Methyl Lysine Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74114-50, BS74114-100\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, ChIP\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IP,1:50 - 1:200   |   ChIP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Methylated\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 90s., Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 3min., Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 10s., Immunoprecipitation analysis of 1000ug extracts of HeLa cells using 20ug Pan Mono-Methyl lysine antibody. Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K9  at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 17-90kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to a sequence containing monomethylatedK.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Methylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892001706163,"sku":"BS74114-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892001738931,"sku":"BS74114-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_30c05e15-b8a1-4072-88e5-f1cb7854476b.jpg?v=1702469004","url":"https:\/\/afsbio.com\/products\/pan-mono-methyl-lysine-polyclonal-antibody-bs74114","provider":"AFSBio Inc.","version":"1.0","type":"link"}