{"product_id":"pan-nitrific-y-polyclonal-antibody-bs74125","title":"Pan-Nitrific-Y Polyclonal Antibody-BS74125","description":"\u003cp\u003ePan-Nitrific-Y Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74125-50, BS74125-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Other (Wide Range)\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Other Modified\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 10-250KDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to a sequence containing myristylatedK.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Other Modified\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using pan-Nitrific-Y antibody at 1:1000 dilution. HeLa and C6 cells were treated by Perxynitratefor 1 hours.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 1s., Western blot analysis of extracts of NIH\/3T3 cells, using pan-Nitrific-Y antibody at 1:1000 dilution. NIH3T3 cells were treated by Perxynitratefor 3 hours.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 10s.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892339249331,"sku":"BS74125-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892339282099,"sku":"BS74125-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b24ec330-27d6-4f19-bfe9-3ee6a48843b1.jpg?v=1702483286","url":"https:\/\/afsbio.com\/products\/pan-nitrific-y-polyclonal-antibody-bs74125","provider":"AFSBio Inc.","version":"1.0","type":"link"}