{"product_id":"pan-phospho-tyrosine-polyclonal-antibody-bs79455","title":"Pan Phospho - Tyrosine Polyclonal Antibody - BS79455","description":"\u003cp\u003ePan Phospho-Tyrosine Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79455-50, BS79455-100\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Other (Wide Range)\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tyrosine phosphorylation (pTyr), much of which occurred on localized multiple sites, initiates cellular signaling, governs cellular functions, and its dysregulation is implicated in many diseases, especially cancers.  pTyr-specific sensing is of great significance for understanding disease states and developing targeted anticancer drugs.\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb at 1:1000 dilution.Jurkat cells were treated by Pervanadate  at 37℃ for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates\/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s., Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine  at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight., Immunofluorescence analysis of NIH\/3T3 cells using Pan Phospho-Tyrosine  at dilution of 1:100. Blue: DAPI for nuclear staining.NIH\/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 20-70KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S \u0026amp;T.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892001312947,"sku":"BS79455-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892001345715,"sku":"BS79455-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_154023db-fbba-459f-b830-c0985fc12ed4.jpg?v=1702468999","url":"https:\/\/afsbio.com\/products\/pan-phospho-tyrosine-polyclonal-antibody-bs79455","provider":"AFSBio Inc.","version":"1.0","type":"link"}