{"product_id":"pan-tri-methyl-lysine-rabbit-pab-a20145","title":"pan-Tri-Methyl lysine Rabbit pAb - A20145","description":"\u003cp\u003epan-Tri-Methyl lysine Rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: A20145-20, A20145-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs). Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.\u003c\/p\u003e\n\n\u003cp\u003eApplications: Methylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP, ELISA, ChIP\u003c\/p\u003e\n\n\u003cp\u003eCross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)\u003c\/p\u003e \n\n\u003cp\u003eObserved Molecular Weight: 15-74kDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003ePurity: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at -20℃. Avoid freeze \/ thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis),  pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eRecommended Dilutions: WB, 1:500 - 1:1000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg\/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 3μg antibody for 5μg-10μg of Chromatin\u003c\/p\u003e\n\n\u003cp\u003eNCBI Alias: pan Kme3\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ABclonal","offers":[{"title":"20uL","offer_id":45189373067443,"sku":"A20145-20","price":179.2,"currency_code":"CAD","in_stock":true},{"title":"100uL","offer_id":45189373100211,"sku":"A20145-100","price":515.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_3dca99f0-1f1b-4f2a-8611-cff76d85fdbf.png?v=1766425693","url":"https:\/\/afsbio.com\/products\/pan-tri-methyl-lysine-rabbit-pab-a20145","provider":"AFSBio Inc.","version":"1.0","type":"link"}