{"product_id":"pan-ubiquitin-remnant-motif-k-ε-gg-polyclonal-antibody-bs74124","title":"Pan-Ubiquitin Remnant Motif (K-ε-GG) Polyclonal Antibody-BS74124","description":"\u003cp\u003ePan-Ubiquitin Remnant Motif (K-ε-GG) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74124-50, BS74124-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-?-Gly-Gly (K-?-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal –RGG sequence on ubiquitin (K-ε-GG). This Ubiquitin K-ε-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Other Modified\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 15-100KDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to a sequence containing ubiquitinated Motif(K-ε-GG)\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Other Modified\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using pan-Ubiquitin Remnant Motif antibody at 1:1000 dilution. HeLa cells were treated by MG132 at 37℃ for 90 minutes.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 180s.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892336529587,"sku":"BS74124-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892336562355,"sku":"BS74124-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6d8f8a47-2530-4468-9bae-8894918c9d81.jpg?v=1702483231","url":"https:\/\/afsbio.com\/products\/pan-ubiquitin-remnant-motif-k-%ce%b5-gg-polyclonal-antibody-bs74124","provider":"AFSBio Inc.","version":"1.0","type":"link"}