{"product_id":"papola-polyclonal-antibody-bs61818","title":"PAPOLA Polyclonal Antibody - BS61818","description":"\u003cp\u003ePAPOLA Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS61818-50, BS61818-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly(A) site, followed by synthesis of the poly(A) tail at the 3' end of the up-stream cleavage product. The poly(A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecμlar masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Poly(A) polymerase alpha, PAP-alpha, Polynucleotide adenylyltransferase alpha, PAPOLA, PAP\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human PAPOLA.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PAPOLA Polyclonal Antibody detects endogenous levels of PAPOLA protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 100 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P51003\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45938440011955,"sku":"BS61818-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45938440044723,"sku":"BS61818-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_7d54f7f7-895a-4faa-805b-93c7ca9b170c.jpg?v=1788296274","url":"https:\/\/afsbio.com\/products\/papola-polyclonal-antibody-bs61818","provider":"AFSBio Inc.","version":"1.0","type":"link"}