{"product_id":"partially-humanized-recombinant-antibody-to-hcv-subtype-1b-ns5b-protein-c2-424-100","title":"Partially Humanized Recombinant Antibody to HCV Subtype 1B NS5B Protein - C2-424-100","description":"\u003cp\u003ePartially Humanized Recombinant Antibody to HCV Subtype 1B NS5B Protein\u003c\/p\u003e\n\n\u003cp\u003eSize: 100 µg\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: C2-424-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: HCV 1b NS5B\u003c\/p\u003e\n\n\u003cp\u003eImmunogen Description: Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q9WMX2\u003c\/p\u003e\n\n\u003cp\u003eClonality: Partially humanized monoclonal\u003c\/p\u003e\n\n\u003cp\u003eClone: 9A2\u003c\/p\u003e\n\n\u003cp\u003eClass: IgG1\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA, WB, IF\u003c\/p\u003e\n\n\u003cp\u003eProtocol: ELISA titer 1:1000; Western immunoblotting, reduced conditions; dilution 1:1000; Immunofluorescense 1:1000\u003cbr\u003e\nMonoclonal antibody working titer has to be established practically for each particular antigen and assay format.\u003c\/p\u003e\n\n\u003cp\u003ePurification: Protein G purification\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS pH 7.4, with 0.1% sodium azide\u003c\/p\u003e\n\n\u003cp\u003eShipping: This product is shipped in non-frozen liquid form in ambient conditions\u003c\/p\u003e\n\n\u003cp\u003eStorage: Store at - 20  to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Icosagen","offers":[{"title":"Default Title","offer_id":44502414524595,"sku":"C2-424-100","price":1195.35,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Icosagen_f41f7084-39bf-4bde-a22e-311328d9fd83.png?v=1741969655","url":"https:\/\/afsbio.com\/products\/partially-humanized-recombinant-antibody-to-hcv-subtype-1b-ns5b-protein-c2-424-100","provider":"AFSBio Inc.","version":"1.0","type":"link"}