pCDNA3.1-BioID2-HA 10μg (Plasmid)

pCDNA3.1-BioID2-HA 10μg (Plasmid)

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Description: pCDNA3.1-BioID2-HA

Format: Liquid; TAE buffer, pH 8.3

Concentration: 200 ng/μl

Volume: 50μl (10μg)

Shipping: On ice

 

VECTOR INFORMATION

Vector: pcDNA 3.1

Size (bp): 6113

Type: Mammalian Expression

Promoter: CMV

Selectable markers: Neomycin (G418 selectable)

Tags: C-terminal Myc tag

Fusions: C-terminal humanized A. aeolicus BioID2 with R40G

 

FOR TRANSFORMATION

Resistance: Amp

Growth Temp: 37°C

Growth Strain: DH5alpha

 

REFERENCES

  1. Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
  2. Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biology (in press) 2016