{"product_id":"pcif1-polyclonal-antibody-bs78221","title":"PCIF1 Polyclonal Antibody-BS78221","description":"\u003cp\u003ePCIF1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS78221-50, BS78221-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9H4Z3\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:100   |   IP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cap-specific adenosine methyltransferase that catalyzes formation of N(6,2'-O-dimethyladenosine cap (m6A(m by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII via interaction with POLR2A and mediates formation of m6A(m co-transcriptionally.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using PCIF1 antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Immunohistochemistry of paraffin-embedded rat brain using PCIF1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug PCIF1 antibody. Western blot was performed from the immunoprecipitate using PCIF1 antibody at a dilution of 1:1000.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PCIF1; C20orf67; PPP1R121; PDX1 C-terminal inhibiting factor 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human PCIF1(NP_071387.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047662682291,"sku":"BS78221-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047662715059,"sku":"BS78221-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_500b17fc-edc9-4be4-a19f-680708aabbbf.jpg?v=1707827009","url":"https:\/\/afsbio.com\/products\/pcif1-polyclonal-antibody-bs78221","provider":"AFSBio Inc.","version":"1.0","type":"link"}