{"product_id":"pcna-polyclonal-antibody-e-ab-18205","title":"PCNA Polyclonal Antibody - E-AB-18205","description":"\u003cp\u003ePCNA Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-18205-60, E-AB-18205-120, E-AB-18205-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: PCNA\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna\/cyclin; PCNA; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P12004\u003c\/p\u003e\n\n\u003cp\u003eBackground: Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 0.7 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Fusion protein of human PCNA\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Antigen affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 29 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43143877656755,"sku":"E-AB-18205-60","price":200.2,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43143877689523,"sku":"E-AB-18205-120","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43143877722291,"sku":"E-AB-18205-200","price":558.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_137bdfc8-1f84-46e2-ae39-0c0ca42b74e7.png?v=1710242937","url":"https:\/\/afsbio.com\/products\/pcna-polyclonal-antibody-e-ab-18205","provider":"AFSBio Inc.","version":"1.0","type":"link"}