{"product_id":"pdh-e1-alpha-monoclonal-antibody-bt-mca1012","title":"PDH-E1 Alpha Monoclonal Antibody - BT-MCA1012","description":"\u003cp\u003ePDH-E1 Alpha Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: BT-MCA1012-50, BT-MCA1012-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Cancer; Metabolism\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: PDHA1\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF, ICC\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human,Mouse,Rat,Bovine,Dog,Pig\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eUniProt Accession: Human: P08559; Mouse: P35486; Rat: P26284\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form; mitochondrial; PDHE1-A type I\u003c\/p\u003e\n\n\u003cp\u003eGeneID: Human: 5160; Mouse: 18597\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"50μL","offer_id":45238743335091,"sku":"BT-MCA1012-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45238743367859,"sku":"BT-MCA1012-100","price":420.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_4845b96b-2e35-47ce-a5a1-c6625f8904d4.png?v=1769384304","url":"https:\/\/afsbio.com\/products\/pdh-e1-alpha-monoclonal-antibody-bt-mca1012","provider":"AFSBio Inc.","version":"1.0","type":"link"}