{"product_id":"pdha1-monoclonal-antibody-mb66491","title":"PDHA1 Monoclonal Antibody - MB66491","description":"\u003cp\u003ePDHA1 Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66491-50, MB66491-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix . This complex is comprised of three enzymes: pyruvate dehydrogenase , dihydrolipoamide acetyltransferase, and dihydrolipoamide dehydrogenase . Pyruvate dehydrogenase consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration, and early death .~Pyruvate dehydrogenase kinase 1 phosphorylates pyruvate dehydrogenase α1 subunit at Ser293 to inactivate its activity . This phosphorylation contributes to the tumor metabolic reprogramming toward glycolysis in hypoxia by inhibiting the citric acid cycle .\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: PHE1A, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial, PDHE1-A type I\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of PDHA1 protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 43 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Warburg Effect\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P08559\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946405093555,"sku":"MB66491-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946405126323,"sku":"MB66491-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/pdha1-monoclonal-antibody-mb66491","provider":"AFSBio Inc.","version":"1.0","type":"link"}