{"product_id":"pdk2-r291-polyclonal-antibody-bs3913","title":"PDK2 (R291) Polyclonal Antibody-BS3913","description":"\u003cp\u003ePDK2 (R291) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS3913-50, BS3913-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q15119\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB 1:500 - 1:1000 \u003cbr\u003e\nIHC 1:50 - 1:100 \u003cbr\u003e\nIP 1:10 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PDK2 (R291) polyclonal antibody detects endogenous levels of PDK2 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 46 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PDK2 (R291) polyclonal antibody at 1:500 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: L02 whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: The brain tissue lysate of Mouse (40ug)\u003cbr\u003e\n Lane 4: The brain tissue lysate of Rat (40ug)\u003cbr\u003e\n Lane 5: The liver tissue lysate of Mouse (40ug)\u003cbr\u003e\n Lane 6: The liver tissue lysate of Rat (40ug), Immunoprecipitation of the Brain tissue lysate of Rat using PDK2 (R291) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using PDK2 (R291) pAb #BS3628. “↑”(supernatant); “↓”(deposition)\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; PDHK2; PDK2; PDK2; Pyruvate dehydrogenase kinase isoform 2; Pyruvate dehydrogenase kinase; isozyme 2; Pyruvate dehydrogenase lipoamide kinase isozyme 2; mitochondrial\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 260-322 of Human PDK2.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989554270387,"sku":"BS3913-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989554303155,"sku":"BS3913-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b9da7949-60c8-4789-8430-5a4157a602bf.jpg?v=1706095269","url":"https:\/\/afsbio.com\/products\/pdk2-r291-polyclonal-antibody-bs3913","provider":"AFSBio Inc.","version":"1.0","type":"link"}