{"product_id":"pdlim1-polyclonal-antibody-bs62275","title":"PDLIM1 Polyclonal Antibody-BS62275","description":"\u003cp\u003ePDLIM1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62275-50, BS62275-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O00151\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB IHC IP IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000 IHC:1:50~1:200 IP:1:50~1:200 IF:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: CLP36, also known as PDLIM1, CLIM1 or Elfin, is a 329 amino acid cytoplasmic protein that associates with Actin stress fibers at the cytoskeleton. Expressed at high levels in skeletal muscle and heart and at lower levels in colon, small intestine, spleen, lung, placenta, kidney, liver, thymus and pancreas, CLP36 functions as a cytoskeletal protein that is thought to act as an adaptor, bringing target proteins to the cytoskeleton. Specifically, CLP36 interacts with Clik1 (a kinase) and recruits Clik1 to α-actinin-1, thereby facilitating the association of Clik1 with Actin stress fibers. CLP36 contains one PDZ domain and one LIM zinc-binding domain through which it conveys its protein-protein binding capabilities. Human CLP36 shares 88% sequence similarity with its rat counterpart, suggesting a conserved function between species.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PDLIM1 polyclonal antibody detects endogenous levels of PDLIM1 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PDLIM1 polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:HEK293T whole cell lysate\u003cbr\u003e\nLaneB:Hela whole cell lysate\u003cbr\u003e\nLaneC:H1688 whole cell lysate\u003cbr\u003e\nLaneD:The Heart tissue lysate of Mouse\u003cbr\u003e\nLaneE:The Muscle tissue lysate of Mouse\u003cbr\u003e\nLaneF:The Heart tissue lysate of Rat\u003cbr\u003e\nLaneG:The Muscle tissue lysate of Rat\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 36 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PDZ and LIM domain protein 1; C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36; PDLIM1; CLIM1; CLP36\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human PDLIM1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910440980659,"sku":"BS62275-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910441013427,"sku":"BS62275-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b0f5b760-d258-4e76-8446-65777f61b6db.jpg?v=1703092405","url":"https:\/\/afsbio.com\/products\/pdlim1-polyclonal-antibody-bs62275","provider":"AFSBio Inc.","version":"1.0","type":"link"}