{"product_id":"perk-phospho-thr981-rabbit-polyclonal-antibody-es1527","title":"PERK (phospho Thr981) Rabbit Polyclonal Antibody - ES1527","description":"\u003cp\u003ePERK (phospho Thr981) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1527-50, ES1527-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: approximately 7-10 business days, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],\u003c\/p\u003e \n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: IF, WB, IHC, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human PEK\/PERK around the phosphorylation site of Thr981. AA range:947-996\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: PERK,\u003c\/p\u003e \n\n\u003cp\u003eSpecificity: Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.\u003c\/p\u003e\n\n\u003cp\u003eDilution: IF: 1:50-200 WB 1:500-2000 , Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e \n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Ubiquitous. A high level expression is seen in secretory tissues.\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 130kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Mitophagy - animal, Autophagy - animal, Protein processing in endoplasmic reticulum, Apoptosis, Non-alcoholic fatty liver disease, Alzheimer disease, Parkinson disease, Amyotrophic lateral sclerosis, Prion disease, Pathways of neurodegeneration - multiple diseases, Hepatitis C, Measles, Herpes simplex virus 1 infection, Lipid and atherosclerosis\u003c\/p\u003e\n\n\u003cp\u003eFunction: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein. Disease: Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities. Domain: The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5\/BIP. enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5\/BIP and oligomerization, resulting in transautophosphorylation and kinase act\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Endoplasmic reticulum membrane, Single-pass type I membrane protein.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: EIF2AK3\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Eukaryotic translation initiation factor 2-alpha kinase 3\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 9451\u003c\/p\u003e\n\n\u003cp\u003eHuman SwissProt Number: Q9NZJ5\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q9Z2B5\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 29702\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q9Z1Z1\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":45825501692083,"sku":"ES1527-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45825501724851,"sku":"ES1527-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk-biotechnology-logo-product.jpg?v=1786476023","url":"https:\/\/afsbio.com\/products\/perk-phospho-thr981-rabbit-polyclonal-antibody-es1527","provider":"AFSBio Inc.","version":"1.0","type":"link"}