{"product_id":"perk-polyclonal-antibody-bz16373","title":"PERK Polyclonal Antibody-BZ16373","description":"\u003cp\u003ePERK Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16373-50, BZ16373-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9NZJ5\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: ICC\/IF, WB, IHC-F, IHC-P, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200  ELISA: 1\/10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha\/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha\/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 125 kDa; Observed MW: 125 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of PERK in MCF-7 lysates using PERK antibody. The Lane on the right is blocked with the synthesized peptide., Immunofluorescence analysis of PERK in Hela using PERK antibody, Immunohistochemistry analysis of paraffin-embedded mouse lung tissue using PERK antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human EIF2AK3. AA range:947-996\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993832886451,"sku":"BZ16373-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993832919219,"sku":"BZ16373-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5ab18e09-44ec-41bb-a7f1-ef9cfab8297e.jpg?v=1706184060","url":"https:\/\/afsbio.com\/products\/perk-polyclonal-antibody-bz16373","provider":"AFSBio Inc.","version":"1.0","type":"link"}