{"product_id":"pgls-polyclonal-antibody-bs5852","title":"PGLS Polyclonal Antibody-BS5852","description":"\u003cp\u003ePGLS Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS5852-50, BS5852-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: 6-phosphogluconolactonase; 6PGL\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O95336\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: PGLS (6-phosphogluconolactonase), also known as 6PGL, is a 258 amino acid protein that belongs to the glucosamine\/galactosamine-6-phosphate isomerase family and the 6-phosphogluconolactonase subfamily. Localizing to cytoplasm, PGLS is a particularly active enzyme that catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate, which is the second step of the pentose phosphate pathway. Highly conserved, PGLS shares 33% to 37% sequence similarity with yeast Sol1, Sol2, Sol3 and Sol4, 26% similarity with the C-terminal portion of human H6PD, 20% to 25% similarity with bacterial devB proteins and 17% similarity with human GNPDA1. PGLS erythrocyte deficiency, an autosomal dominant disorder, in conjunction with G6PD deficiency, may play a role in hemolytic anemia.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PGLS polyclonal antibody detects endogenous levels of PGLS protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 28 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PGLS (A226) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: HCT116 whole cell lysate(40ug)\u003cbr\u003e\nLane 2: Panc1 whole cell lysate(40ug)\u003cbr\u003e\nLane 3: HEK293T whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 213-258 of Human PGLS.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911819792563,"sku":"BS5852-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911819825331,"sku":"BS5852-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_1c956570-5615-418a-89a0-0744b6e9c78c.jpg?v=1703179998","url":"https:\/\/afsbio.com\/products\/pgls-polyclonal-antibody-bs5852","provider":"AFSBio Inc.","version":"1.0","type":"link"}