{"product_id":"phb-rabbit-monoclonal-antibody-bs9857m","title":"PHB Rabbit Monoclonal Antibody-BS9857M","description":"\u003cp\u003ePHB Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9857M-50, BS9857M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P35232\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat, Zebra fish\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC, IHC, IP,  FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000\u003cbr\u003e\nICC: 1:50-1:200\u003cbr\u003e\nIHC: 1:200-1:500 \u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of PHB and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 30 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PHB Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: 293 whole cell lysate\u003cbr\u003e\n Lane 2: Jurkat whole cell lysate\u003cbr\u003e\n Lane 3: The kidney tissue lysate of Mouse\u003cbr\u003e\n Lane 4: HepG2 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin., ICC staining Prohibitin in NIH\/3T3 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS., Flow cytometric analysis of HepG2 cells with PHB antibody at 1\/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Prohibitin\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058777424051,"sku":"BS9857M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058777456819,"sku":"BS9857M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9250faa6-c12c-4b0f-857d-9cdd0fcf105f.jpg?v=1708172192","url":"https:\/\/afsbio.com\/products\/phb-rabbit-monoclonal-antibody-bs9857m","provider":"AFSBio Inc.","version":"1.0","type":"link"}