{"product_id":"phospho-akt-ser473-8a9-monoclonal-antibody-mb3262","title":"Phospho-AKT (Ser473) (8A9) Monoclonal Antibody-MB3262","description":"\u003cp\u003ePhospho-AKT (Ser473) (8A9) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB3262-50, MB3262-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P31749\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG1\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 56 kDa; Observed MW: 60 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Phospho-AKT in PC-3 lysates using Phospho-AKT antibody., Immunohistochemistry analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: AKT1; PKB; RAC; RAC-alpha serine\/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide conjugated to KLH.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42970466681011,"sku":"MB3262-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42970466713779,"sku":"MB3262-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_3ebc5138-72dc-4a6b-854a-145104d3adff.jpg?v=1705525700","url":"https:\/\/afsbio.com\/products\/phospho-akt-ser473-8a9-monoclonal-antibody-mb3262","provider":"AFSBio Inc.","version":"1.0","type":"link"}