{"product_id":"phospho-ampk-alpha1-2-thr183-172-polyclonal-antibody-e-ab-21121","title":"Phospho-AMPK alpha1\/2 (Thr183\/172) Polyclonal Antibody - E-AB-21121","description":"\u003cp\u003ePhospho-AMPK alpha1\/2 (Thr183\/172) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 60μL, 120μL, 200μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-AB-21121-60, E-AB-21121-120, E-AB-21121-200\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: AMPKα1\/2 (phospho Thr183\/172)\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonym: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;\u003c\/p\u003e \n\n\u003cp\u003eResearch Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction\u003c\/p\u003e\n\n\u003cp\u003eConjugation: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eSpecies Reactivity: Human, Mouse, Rat, Monkey\u003c\/p\u003e\n\n\u003cp\u003eApplication: WB, IHC-p, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n \n\n\u003cp\u003eUNIProt ID: Q13131,P54646\u003c\/p\u003e\n\n\u003cp\u003eBackground: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/mL\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized peptide derived from human AMPKα1\/2 around the phosphorylation site of  Thr183\/172\u003c\/p\u003e\n\n\u003cp\u003eBuffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4\u003c\/p\u003e\n\n\u003cp\u003ePurification Method: Affinity purification\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000\u003c\/p\u003e\n\n\u003cp\u003eCalculated MW: 62kDa\u003c\/p\u003e\n\n\u003cp\u003eObserved MW: 63kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C\/One year. Avoid freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"60μL","offer_id":43146003513523,"sku":"E-AB-21121-60","price":200.2,"currency_code":"CAD","in_stock":true},{"title":"120μL","offer_id":43146003546291,"sku":"E-AB-21121-120","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"200μL","offer_id":43146003579059,"sku":"E-AB-21121-200","price":558.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/logo_new_5762a40f-c3f8-4bc3-8038-3cef034f14c3.png?v=1710289610","url":"https:\/\/afsbio.com\/products\/phospho-ampk-alpha1-2-thr183-172-polyclonal-antibody-e-ab-21121","provider":"AFSBio Inc.","version":"1.0","type":"link"}