{"product_id":"phospho-b-raf-s445-polyclonal-antibody-bs6338","title":"Phospho-B-Raf-S445 Polyclonal Antibody-BS6338","description":"\u003cp\u003ePhospho-B-Raf-S445 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS6338-50, BS6338-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P15056\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a protein belonging to the raf\/mil family of serine\/threonine protein kinases. This protein plays a role in regulating the MAP kinase\/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb at 1:2000 dilution or BRAF antibody. Raji cells were treated by PMA\/TPA for 30 minutes. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% BSA. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 10s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 100kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: BRAF; B-RAF1; B-raf; BRAF1; NS7; RAFB1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S445 of human BRAF(NP_004324.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939212497075,"sku":"BS6338-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939212529843,"sku":"BS6338-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_aaf07bc2-cf81-4f46-8110-0672bcd61e5d.jpg?v=1704545213","url":"https:\/\/afsbio.com\/products\/phospho-b-raf-s445-polyclonal-antibody-bs6338","provider":"AFSBio Inc.","version":"1.0","type":"link"}