{"product_id":"phospho-b23-thr199-polyclonal-antibody-bz16486","title":"Phospho-B23 (Thr199) Polyclonal Antibody-BZ16486","description":"\u003cp\u003ePhospho-B23 (Thr199) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16486-50, BZ16486-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P06748\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-F, IHC-P, ICC\/IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200  ELISA: 1\/10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53\/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic\/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2\/PKR and suppresses apoptosis through inhibition of EIF2AK2\/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2\/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 33 kDa; Observed MW: 33 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide, using NPM (Phospho-Thr19antibody, Immunofluorescence analysis of Phospho-B23 in HeLa treated with EGF using Phospho-B23 antibody. The picture on the right is blocked with the Phospho- peptide., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-B23 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-B23 in HepG2 lysates using Phospho-B23 antibody. The Lane on the right is blocked with the synthesized peptide.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: B23; NPM\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43055904424115,"sku":"BZ16486-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43055904456883,"sku":"BZ16486-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e15cf3a5-2f34-4760-9e2b-ce504a43d0a1.jpg?v=1708086407","url":"https:\/\/afsbio.com\/products\/phospho-b23-thr199-polyclonal-antibody-bz16486","provider":"AFSBio Inc.","version":"1.0","type":"link"}