{"product_id":"phospho-brd4-s1070-polyclonal-antibody-bs74213","title":"Phospho-BRD4-S1070 Polyclonal Antibody-BS74213","description":"\u003cp\u003ePhospho-BRD4-S1070 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74213-50, BS74213-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O60885\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine\/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-BRD4-S1070 antibody at 1:1000 dilution. HeLa cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST. \u003cbr\u003e\nDetection: ECL Enhanced Kit. \u003cbr\u003e\nExposure time: 180s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 250KDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eAlternative Name: BRD4; CAP; HUNK1; HUNKI; MCAP; bromodomain containing 4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S1070 of human BRD4(NP_490597.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42938034192563,"sku":"BS74213-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42938034225331,"sku":"BS74213-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e13aedf3-8cf8-47b0-927b-4e9c83703a8f.jpg?v=1704459183","url":"https:\/\/afsbio.com\/products\/phospho-brd4-s1070-polyclonal-antibody-bs74213","provider":"AFSBio Inc.","version":"1.0","type":"link"}