{"product_id":"phospho-cdk1-y15-polyclonal-antibody-bs6342","title":"Phospho-CDK1-Y15 Polyclonal Antibody-BS6342","description":"\u003cp\u003ePhospho-CDK1-Y15 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS6342-50, BS6342-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P06493\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:1000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:100   |   IP,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a member of the Ser\/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1\/S and G2\/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 34kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of NIH\/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution or CDK1 antibody. HeLa cellls were treated by nocodazole at 37℃ for 20 hours or Hydroxyurea at 37℃ for 20 hours.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Western blot analysis of extracts of various cell lines, using Phospho-CDK1-T15 antibody at 1:1000 dilution.HeLa and NIH\/3T3 cells were treated by nocodazole at 37℃ for 20 hours.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 10s., Western blot analysis of extracts of C6 cells, using Phospho-CDK1-T15 antibody at 1:1000 dilution.C6 cells were treated by Nocodazole at 37℃ for 20 hours.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 90s., Immunohistochemistry of paraffin-embedded rat liver using Phospho-CDK1-Y15 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CDK1; CDC2; CDC28A; P34CDC2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around Y15 of human CDK1(NP_001777.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058785026227,"sku":"BS6342-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058785058995,"sku":"BS6342-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9dda391b-8c95-486c-9b98-ff49142c2dd6.jpg?v=1708172425","url":"https:\/\/afsbio.com\/products\/phospho-cdk1-y15-polyclonal-antibody-bs6342","provider":"AFSBio Inc.","version":"1.0","type":"link"}