{"product_id":"phospho-cfl1-s3-polyclonal-antibody-bs74132","title":"Phospho-CFL1-S3 Polyclonal Antibody-BS74132","description":"\u003cp\u003ePhospho-CFL1-S3 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74132-50, BS74132-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P23528\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:100   |   IF\/ICC,1:100 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 18kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of NIH\/3T3 cells, using Phospho-CFL1-S3 pAb at 1:1000 dilution or CFL1 antibody. NIH\/3T3 cells were treated by CIP at 37℃ for 1 hour.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 3s., Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-CFL1-S3 antibody. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-CFL1-S3 antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CFL1; CFL; HEL-S-15; cofilin; cofilin-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S3 of human CFL1(NP_005498.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004757049523,"sku":"BS74132-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004757082291,"sku":"BS74132-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_adbc5ee6-a610-43ec-9138-78c8fd2e0b8f.jpg?v=1706445788","url":"https:\/\/afsbio.com\/products\/phospho-cfl1-s3-polyclonal-antibody-bs74132","provider":"AFSBio Inc.","version":"1.0","type":"link"}