{"product_id":"phospho-eef2k-s366-polyclonal-antibody-bs74189","title":"Phospho-EEF2K-S366 Polyclonal Antibody-BS74189","description":"\u003cp\u003ePhospho-EEF2K-S366 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74189-50, BS74189-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O00418\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 110kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody at 1:1000 dilution. Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by PMA\/TPA for 15 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 15s., Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody at 1:1000 dilution.NIH\/3T3 and C6 cells were treated by CIP at 37℃ for 1 hour or PMA\/TPA at 37℃ for 30 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 30s., Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 pAb at 1:1000 dilution or EEF2K antibody. Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by PMA\/TPA for 15 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 15s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EEF2K; HSU93850; eEF-2K\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S366 of human EEF2K(NP_037434.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004757835955,"sku":"BS74189-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004757868723,"sku":"BS74189-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_c908e724-ec25-482b-8fcc-3a9c81cd979c.jpg?v=1706445800","url":"https:\/\/afsbio.com\/products\/phospho-eef2k-s366-polyclonal-antibody-bs74189","provider":"AFSBio Inc.","version":"1.0","type":"link"}