{"product_id":"phospho-egfr-t669-polyclonal-antibody-bs6351","title":"Phospho-EGFR-T669 Polyclonal Antibody-BS6351","description":"\u003cp\u003ePhospho-EGFR-T669 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS6351-50, BS6351-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P00533\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IP,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 200kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody at 1:1000 dilution. A431 cells were treated by EGF for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 10s., Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-T669 pAb. Western blot was performed from the immunoprecipitate using Phospho-EGFR-T669 pAb at a dilution of 1:1000. A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around T669 of human EGFR(NP_005219.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987084710067,"sku":"BS6351-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987084742835,"sku":"BS6351-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_f2d8f743-b3df-44b0-b271-09412932490d.jpg?v=1706008799","url":"https:\/\/afsbio.com\/products\/phospho-egfr-t669-polyclonal-antibody-bs6351","provider":"AFSBio Inc.","version":"1.0","type":"link"}