{"product_id":"phospho-fgfr1-y653-polyclonal-antibody-bs6362","title":"Phospho-FGFR1-Y653 Polyclonal Antibody-BS6362","description":"\u003cp\u003ePhospho-FGFR1-Y653 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS6362-50, BS6362-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11362\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of 293T cells, using Phospho-FGFR1-Y653 pAb at 1:1000 dilution. 293T cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% BSA. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 30s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 120kDa\/145kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated Antibodies\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FGFR1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FLG; FLT-2; FLT2; HBGFR; HH2; HRTFDS; KAL2; N-SAM; OGD; bFGF-R-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around Y653 of human FGFR1(NP_075598.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939212988595,"sku":"BS6362-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939213021363,"sku":"BS6362-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e23769a3-d3ff-434a-a56e-c9e4ddbc3775.jpg?v=1704545231","url":"https:\/\/afsbio.com\/products\/phospho-fgfr1-y653-polyclonal-antibody-bs6362","provider":"AFSBio Inc.","version":"1.0","type":"link"}