{"product_id":"phospho-foxo1-t24-foxo3a-t32-foxo4-t28-polyclonal-antibody-bs79452","title":"Phospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 Polyclonal Antibody-BS79452","description":"\u003cp\u003ePhospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79452-50, BS79452-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q12778\/O43524\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain.  The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 65KDa\/73KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Phospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 antibody at 1:500 dilution.Jurkat cells were treated by Calyculin A at 37℃ for 30 minutes.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat liver using Phospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FOXO1-T24\/FOXO3A-T32\/FOXO4-T28 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FoxO1\/FoxO3a\/FoxO4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around T24 of human FOXO1\/FOXO3A\/FOXO4FOXO1(NP_002006.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058770739379,"sku":"BS79452-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058770772147,"sku":"BS79452-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_aef3b9d4-c53e-4244-abaf-69c474ec5374.jpg?v=1708172056","url":"https:\/\/afsbio.com\/products\/phospho-foxo1-t24-foxo3a-t32-foxo4-t28-polyclonal-antibody-bs79452","provider":"AFSBio Inc.","version":"1.0","type":"link"}