{"product_id":"phospho-gsk3α-s21-polyclonal-antibody-bs74159","title":"Phospho-GSK3α-S21 Polyclonal Antibody-BS74159","description":"\u003cp\u003ePhospho-GSK3α-S21 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74159-50, BS74159-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P49840\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IP,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a multifunctional Ser\/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 51kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin for 15 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA., Western blot analysis of extracts of 293 cells, using Phospho-GSK3α-S21 antibody at 1:1000 dilution or GSK3α antibody. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA., Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3α-S21 pAb. Western blot was performed from the immunoprecipitate using Phospho-GSK3α-S21 pAb at a dilution of 1:1000. 293T cells were treated by Insulin at 37℃ for 10 minutes after serum-starvation overnight.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: GSK3 alpha; GSK3A\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S21 of human GSK3α(NP_063937.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004757344435,"sku":"BS74159-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004757377203,"sku":"BS74159-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_70950253-c3cc-4fb4-bca0-5ad65b7d4b38.jpg?v=1706445795","url":"https:\/\/afsbio.com\/products\/phospho-gsk3%ce%b1-s21-polyclonal-antibody-bs74159","provider":"AFSBio Inc.","version":"1.0","type":"link"}