{"product_id":"phospho-histone-h2a-s129-polyclonal-antibody-bs79470","title":"Phospho-Histone H2A-S129 Polyclonal Antibody-BS79470","description":"\u003cp\u003ePhospho-Histone H2A-S129 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79470-50, BS79470-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04911\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S129 of saccharomyces cerevisiae Histone H2A(NP_010511.3?).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H2A-S129 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-Histone H2A-S129 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892363137203,"sku":"BS79470-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892363169971,"sku":"BS79470-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_142ac3f9-35a8-4611-a78a-7ebec02742fd.jpg?v=1702483860","url":"https:\/\/afsbio.com\/products\/phospho-histone-h2a-s129-polyclonal-antibody-bs79470","provider":"AFSBio Inc.","version":"1.0","type":"link"}