{"product_id":"phospho-hsp27-hspb1-s78-polyclonal-antibody-bs79359","title":"Phospho-HSP27\/HSPB1-S78 Polyclonal Antibody-BS79359","description":"\u003cp\u003ePhospho-HSP27\/HSPB1-S78 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79359-50, BS79359-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04792\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:100   |   IF\/ICC,1:100 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 28kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-HSP27\/HSPB1-S78 antibody at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes or treated by serum-starvation overnight or treated by Anisomycin for 30 minutes after serum-starvation overnight.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 30s., Western blot analysis of extracts of HeLa cells, using Phospho-HSP27\/HSPB1-S78 pAb at 1:1000 dilution or HSP27\/HSPB1 antibody. HeLa cells were treated by UV at room temperature for 15-30 minutes.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 30s., Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-HSP27\/HSPB1-S78 antibody. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using Phospho-HSP27\/HSPB1-S78 antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HSPB1; CMT2F; HEL-S-102; HMN2B; HS.76067; HSP27; HSP28; Hsp25; SRP27\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S78 of human HSP27\/HSPB1(NP_001531.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058769264819,"sku":"BS79359-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058769297587,"sku":"BS79359-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2db2ac63-0c5a-4e22-adf2-56e83771df78.jpg?v=1708172023","url":"https:\/\/afsbio.com\/products\/phospho-hsp27-hspb1-s78-polyclonal-antibody-bs79359","provider":"AFSBio Inc.","version":"1.0","type":"link"}