{"product_id":"phospho-p90rsk-rsk1-rps6ka1-s380-polyclonal-antibody-bs79402","title":"Phospho-p90Rsk\/RSK1\/RPS6KA1-S380 Polyclonal Antibody-BS79402","description":"\u003cp\u003ePhospho-p90Rsk\/RSK1\/RPS6KA1-S380 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79402-50, BS79402-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q15418\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IP,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine\/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 90kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Phospho-p90Rsk\/RSK1\/RPS6KA1-S380 antibody at 1:1000 dilution or RPS6KA1 antibody. HeLa cells were treated by PMA\/TPA at 37℃ for 15 minutes after serum-starvation overnight. \u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Western blot analysis of extracts of various cell lines, using Phospho-p90Rsk\/RSK1\/RPS6KA1-S380 antibody at 1:1000 dilution. NIH\/3T3 cells were treated by PMA\/TPA at 37℃ for 30 minutes after serum-starvation overnight. \u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% BSA.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-p90Rsk\/RSK1\/RPS6KA1-S380 pAb. Western blot was performed from the immunoprecipitate using Phospho-p90Rsk\/RSK1\/RPS6KA1-S380 pAb at a dilution of 1:1000. HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: HU-1; MAPKAPK1A; RSK; RSK1; p90Rsk; RPS6KA1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S380 of human p90Rsk\/RSK1\/RPS6KA1(NP_002944.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004761571507,"sku":"BS79402-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004761604275,"sku":"BS79402-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8ce69f70-3397-4ea6-aafd-33286b93ff5d.jpg?v=1706445860","url":"https:\/\/afsbio.com\/products\/phospho-p90rsk-rsk1-rps6ka1-s380-polyclonal-antibody-bs79402","provider":"AFSBio Inc.","version":"1.0","type":"link"}