{"product_id":"phospho-pkc-θ-y90-rabbit-pab-es20173","title":"Phospho PKC θ (Y90) rabbit pAb - ES20173","description":"\u003cp\u003ePhospho PKC θ (Y90) rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES20173-50, ES20173-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., domain:The C1 domain, containing the phorbol ester\/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain., enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation., function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters., function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production., PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser\/Thr protein kinase family. PKC subfamily., similarity:Contains 1 AGC-kinase C-terminal domain., similarity:Contains 1 C2 domain., similarity:Contains 1 protein kinase domain., similarity:Contains 2 phorbol-ester\/DAG-type zinc fingers., subunit:Interacts with TXNL2\/PICOT., tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.,\u003c\/p\u003e \n\n\u003cp\u003eAlternate Name: Protein kinase C theta type (EC 2.7.11.13; nPKC-theta)\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500-2000; IHC-p 1:50-300\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human; Mouse; Rat\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthesized peptide derived from human Phospho PKC θ (Y90)\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 78kD\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 5588\u003c\/p\u003e\n\n\u003cp\u003eHuman SWISS Prot NO: Q04759\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45202428526771,"sku":"ES20173-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45202428559539,"sku":"ES20173-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_dd607c09-c9c2-4b55-bb42-e1fda12604bc.png?v=1767129002","url":"https:\/\/afsbio.com\/products\/phospho-pkc-%ce%b8-y90-rabbit-pab-es20173","provider":"AFSBio Inc.","version":"1.0","type":"link"}