{"product_id":"phospho-pkc-mu-s744-748-polyclonal-antibody-bs74149","title":"Phospho-PKC mu-S744\/748 Polyclonal Antibody-BS74149","description":"\u003cp\u003ePhospho-PKC mu-S744\/748 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74149-50, BS74149-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q15139\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene is a serine\/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8\/JNK1 and Ras pathway signaling, MAPK1\/3 (ERK1\/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of NIH\/3T3 cells, using Phospho-PKC mu-S744\/748 pAb at 1:1000 dilution or PRKD1 antibody. NIH\/3T3 cells were treated by PMA\/TPA for 30 minutes after serum-starvation overnight. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% BSA. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 5s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 102kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eAlternative Name: CHDED; PKC-MU; PKCM; PKD; PRKCM; PRKD1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around S744 \u0026amp; S748 of mouse PKC mu(NP_032884.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42938030555315,"sku":"BS74149-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42938030588083,"sku":"BS74149-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_12dbce4c-9204-4414-acbe-38ddfb234ad7.jpg?v=1704459087","url":"https:\/\/afsbio.com\/products\/phospho-pkc-mu-s744-748-polyclonal-antibody-bs74149","provider":"AFSBio Inc.","version":"1.0","type":"link"}