{"product_id":"phospho-raf1-ser259-polyclonal-antibody-bz16727","title":"Phospho-Raf1 (Ser259) Polyclonal Antibody-BZ16727","description":"\u003cp\u003ePhospho-Raf1 (Ser259) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16727-50, BZ16727-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04049\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB,IHC-P\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine\/threonine specific protein kinases ERK1 and ERK2.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Phospho-Raf1 in 293TLysates; 293TLysates treated with Okadaic Acid + Calyculin A using Phospho-Raf1 antibody.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 73 kDa; Observed MW: 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RAF1; RAF; RAF proto-oncogene serine\/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthesized peptide derived from human Phospho-Raf1 (S259)\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42935421534387,"sku":"BZ16727-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42935421567155,"sku":"BZ16727-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9c9368ff-eb8d-464e-99a4-404c1e0fcc9d.jpg?v=1704370321","url":"https:\/\/afsbio.com\/products\/phospho-raf1-ser259-polyclonal-antibody-bz16727","provider":"AFSBio Inc.","version":"1.0","type":"link"}