{"product_id":"phospho-rb-ser780-polyclonal-antibody-bz16380","title":"Phospho-Rb (Ser780) Polyclonal Antibody-BZ16380","description":"\u003cp\u003ePhospho-Rb (Ser780) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16380-50, BZ16380-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P06400\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-F, IHC-P, ICC\/IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200  ELISA: 1\/10000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4\/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK\/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 106 kDa; Observed MW: 106 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Phospho-Rb in various lysates using Phospho-Rb antibody., Western blot analysis of Phospho-Rb in KB lysates using Phospho-Rb antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Retinoblastoma antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-Rb in K562 lysates treated with serum 10% using Phospho-Rb antibody. The Lane on the right is blocked with the Phospho- peptide.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43055903670451,"sku":"BZ16380-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43055903703219,"sku":"BZ16380-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_31c41b42-bb31-43fe-82ac-1880a840571e.jpg?v=1708086385","url":"https:\/\/afsbio.com\/products\/phospho-rb-ser780-polyclonal-antibody-bz16380","provider":"AFSBio Inc.","version":"1.0","type":"link"}