{"product_id":"phospho-ser-thr-atm-atr-substrate-polyclonal-antibody-bs79460","title":"Phospho-(Ser\/Thr) ATM\/ATR Substrate Polyclonal Antibody-BS79460","description":"\u003cp\u003ePhospho-(Ser\/Thr) ATM\/ATR Substrate Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79460-50, BS79460-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln.   It also  called SQ or TQ consensus sites.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 38-68KDa\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S \u0026amp;T.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Phospho- ATM\/ATR Substrate pAb at 1:1000 dilution.Jurkat and NIH\/3T3 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP at 30℃ for 1 hour.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 60s.\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892337610931,"sku":"BS79460-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892337643699,"sku":"BS79460-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_48dbcd39-43b1-48a8-b6c2-c74539f787da.jpg?v=1702483252","url":"https:\/\/afsbio.com\/products\/phospho-ser-thr-atm-atr-substrate-polyclonal-antibody-bs79460","provider":"AFSBio Inc.","version":"1.0","type":"link"}