{"product_id":"phospho-serca2-atp2a2-thr484-polyclonal-antibody-bs79473","title":"Phospho-SERCA2\/ATP2A2-Thr484 Polyclonal Antibody-BS79473","description":"\u003cp\u003ePhospho-SERCA2\/ATP2A2-Thr484 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79473-50, BS79473-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P16615\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction\/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Phospho-SERCA2\/ATP2A2-Thr484 antibody at 1:1000 dilution. NIH\/3T3 cells were treated by ATP at 30℃ for 1 hour. C6 cells were treated by ATP at 30℃ for 1 hour. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% BSA. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 1s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 114KkDa\/140kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eAlternative Name: ATP2B; DAR; DD; SERCA2; ATP2A2\/SERCA2; ATP2A2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around T484 of SERCA2\/ATP2A2(NP_733765.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42939194146995,"sku":"BS79473-50","price":415.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42939194179763,"sku":"BS79473-100","price":640.78,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a5f67013-3f1f-47b1-a2f7-c65bd7e800cf.jpg?v=1704544814","url":"https:\/\/afsbio.com\/products\/phospho-serca2-atp2a2-thr484-polyclonal-antibody-bs79473","provider":"AFSBio Inc.","version":"1.0","type":"link"}