{"product_id":"phospho-zap70-y493-polyclonal-antibody-bs79376","title":"Phospho-ZAP70-Y493 Polyclonal Antibody-BS79376","description":"\u003cp\u003ePhospho-ZAP70-Y493 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS79376-50, BS79376-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P43403\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:2000 - 1:5000   |   IHC,1:50 - 1:100   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phosphorylated\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 70KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of Jurkat cells, using Phospho-ZAP70-Y493 antibody at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 1s., Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-ZAP70-Y493 antibody. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of Jurkat cells using Phospho-ZAP70-Y493 Rabbit pAb at dilution of 1:100.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: ZAP70; ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic phosphorylated peptide around Y493 of human ZAP70(NP_001070.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004760948915,"sku":"BS79376-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004760981683,"sku":"BS79376-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_78f02b90-3c73-4ff1-b4b2-46a6ccac6774.jpg?v=1706445852","url":"https:\/\/afsbio.com\/products\/phospho-zap70-y493-polyclonal-antibody-bs79376","provider":"AFSBio Inc.","version":"1.0","type":"link"}