{"product_id":"pi-3-kinase-p85α-rabbit-polyclonal-antibody-es3199","title":"PI 3-kinase p85α Rabbit Polyclonal Antibody - ES3199","description":"\u003cp\u003ePI 3-kinase p85α Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES3199-50, ES3199-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alph\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha. AA range:573-622\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: PI3 Kinase P85α\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: PI 3-kinase p85α Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/5000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle, Isoform 2 and isoform 4 are present in skeletal muscle (at protein level),\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 85kD\u003c\/p\u003e\n\n\u003cp\u003eFields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, Platinum drug resistance, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, cAMP signaling pathway, Chemokine signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Phosphatidylinositol signaling system, Sphingolipid signaling pathway, Phospholipase D signaling pathway, Autophagy - animal, mTOR signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Apoptosis, Longevity regulating pathway, Longevity regulating pathway - multiple species, Cellular senescence, Axon guidance, VEGF signaling pathway, Osteoclast differentiation, Focal adhesion, Signaling pathways regulating pluripotency of stem cells, Platelet activation, Neutrophil extracellular trap formation, Toll-like receptor signaling pathway, C-type lectin receptor signaling pathway, JAK-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, \u0026gt;\u003c\/p\u003e\n\n\u003cp\u003eFunction: Disease: Defects in PIK3R1 are a cause of severe insulin resistance\u003cbr\u003e\nDomain: The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef\u003cbr\u003e\nFunction: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues\u003cbr\u003e\nPTM: Polyubiquitinated in T-cells by CBLB, which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation\u003cbr\u003e\nSimilarity: Belongs to the PI3K p85 subunit family\u003cbr\u003e\nSimilarity: Contains 1 Rho-GAP domain\u003cbr\u003e\nSimilarity: Contains 1 SH3 domain\u003cbr\u003e\nSimilarity: Contains 2 SH2 domains\u003cbr\u003e\nSubunit: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylat\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: nucleus, cytoplasm, cis-Golgi network, cytosol, plasma membrane, cell-cell junction, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA, membrane, perinuclear endoplasmic reticulum membrane,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: PIK3R1\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 18708\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: P26450\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 25513\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: Q63787\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46316468863155,"sku":"ES3199-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46316468895923,"sku":"ES3199-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/pi-3-kinase-p85%ce%b1-rabbit-polyclonal-antibody-es3199","provider":"AFSBio Inc.","version":"1.0","type":"link"}