{"product_id":"pig-baff-cd257-b-cell-activating-factor-elisa-kit-elk8277","title":"Pig BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit - ELK8277","description":"\u003cp\u003ePig BAFF\/CD257 (B-Cell Activating Factor) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8277-48, ELK8277-96, ELK8277-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: CD257; TNFSF13B; BLYS; TALL1; THANK; TNFSF20; ZTNF4; B lymphocyte stimulator; Tumor Necrosis Factor Ligand Superfamily Member 13B; Dendritic cell-derived TNF-like; BAFF\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.057 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig BAFF\/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig BAFF\/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig BAFF\/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig BAFF\/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212418875571,"sku":"ELK8277-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212418908339,"sku":"ELK8277-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200794484915,"sku":"ELK8277-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_2030e873-37a2-4774-bc0c-f98a245bf7f6.png?v=1767064017","url":"https:\/\/afsbio.com\/products\/pig-baff-cd257-b-cell-activating-factor-elisa-kit-elk8277","provider":"AFSBio Inc.","version":"1.0","type":"link"}