{"product_id":"pim1-rabbit-monoclonal-antibody-bs9950m","title":"PIM1 Rabbit Monoclonal Antibody -BS9950M","description":"\u003cp\u003ePIM1 Rabbit Monoclonal Antibody\u003c\/p\u003e \n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9950M-50, BS9950M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11309\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000 \u003cbr\u003e\nICC\/IF: 1:50-1:200\u003cbr\u003e\nIHC: 1:50-1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Pim-1 is a serine\/threonine kinase that cooperates with c-Myc in lymphoid cell transformation. The expression of pim-1 increases during the progression from early to late G1, remaining high at the G1\/S boundary and G2 phases of the cell cycle. Pim-1 is regulated at both the transcriptional and translational level, and it has been shown to be induced by IL-2 stimulation. Pim-1 also plays a role in T cell differentiation, and it has been shown to stimulate c-Myc-mediated apoptosis upstream of caspase-3-like proteases.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of PIM1 and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 45 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of PIM1 Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: BT-20 whole cell lysate\u003cbr\u003e\n Lane 2: HepG2 whole cell lysate\u003cbr\u003e\n Lane 3: SW480 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-PIM1 antibody.Counter stained with hematoxylin., ICC staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/threonine-protein kinase pim-1; Pim1; Pim-1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant antibody.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044035526835,"sku":"BS9950M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044035559603,"sku":"BS9950M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b65b98b6-8dec-497c-8811-1c4a8f39afda.jpg?v=1707738821","url":"https:\/\/afsbio.com\/products\/pim1-rabbit-monoclonal-antibody-bs9950m","provider":"AFSBio Inc.","version":"1.0","type":"link"}